Typically, as one gets older, their waistline changes and this is because fat cells in the abdominal area of your body are extremely sensitive to cortisol fluctuations. High cortisol leads to fat accumulation in the abdomen. Cortisol is active, cortisone is inactive and DUTCH evaluates both and finds out which hormone is predominant. A waking urine sample allows represents the entire night! The next sample is taken 2 hours later and you should see a surge in active cortisol. A total of 4 samples allows DUTCH to give you your 24 hour cycle with peaks and troughs. Evaluating the actual metabolites of cortisol can give you an idea (free cortisol being low, and metabolized cortisol is relatively high, this means there is a lot of cortisol clearance which is common in obesity, hyperthyroidism and more).
There is also a strong correlation between dopamine and stress. During chronic stress, your body is initially flooded with cortisol, resulting in a dopamine depletion. It turns out that your body will basically replace dopamine with the excess cortisol. This results in anxiety, depression, memory loss, heart disease, higher risk of auto-immune conditions and low pleasure. Pills don't fix this, evaluating cortisol could.